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Figure 3.
ptf1a/p48-targeted X. laevis G0 tadpoles showed reduction of pdip expression. Whole-mount in situ hybridization analyses of pdip expression (stage 42). Ventral-lateral view, head to the left. (A, B) Wild type X. laevis tadpoles. (C, E) Severe reduction. (D, F) Mild reduction. (G, I) Severe reduction. (H, J) Mild reduction. (K, N) Almost complete absence. (L, O) Severe reduction. (M, P) Mild reduction. |
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Figure 1. pdip expression is undetectable in X. tropicalis ptf1a/p48-null mutant tadpoles. Whole-mount in situ hybridization analysis of pdip expression in F2 X. tropicalis ptf1a/p48 mutant tadpoles (stage 43). Ventral-lateral view, head to the left. (A, C) About 22% homozygous tadpoles showed complete absence of pdip expression. (B, D) The remainder uniformly showed strong pdip expression, suggesting normal pancreas development in ptf1a/p48 heterozygous tadpoles. |