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Figure 3. Xenopus appl1 knockdown leads to a specific inhibition of pancreas and SD marker gene expression during tadpole stages of development. A: Whole mount in situ hybridization analysis revealed that XPDIp expression was significantly suppressed in Mo1-injected stage-42 embryos and inhibition levels were correlated with the severeness of the edema. B: Whole mount in situ hybridization analyses of XHex, XlHbox8, Foxa1, Ptf1a/p48, and XPDIp expression in Mo1, MoC, Mo1/APPL1, and Mo1/akt2-injected embryos. C:Insulin, insm1, Ptf1a/p48, and XlHbox8 expression at tail bud stage was not significantly altered upon Mo1 injection. The injection was done with 40 ng of MoC, 40 ng of Mo1, 40 ng of Mo1 plus 1 ng of human APPL1 mRNA, or 40 ng of Mo1 plus 1 ng of akt2 mRNA into all four vegetal blastomeres of 8-cell-stage embryos.Download figure to PowerPoint |
