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Fig. 5. Blocking RA signalling specifically in either the endoderm or the mesoderm of whole embryos interferes with pancreas specification. To block RA signalling specifically in the endoderm, DN-RARα2 was injected into the vegetal pole of 8-cell stage embryos together with the lineage tracer β-gal. In order to block RA signalling in the mesoderm, DN-RARα2 and β-gal were co-injected into the marginal zone of the two dorsal blastomeres in 4-cell stage embryos. The injected embryos were cultivated until control siblings had reached stage 36 or stage 39, and subjected to β-gal staining before whole mount in situ hybridization was carried out. (panels 1–3) Lateral view of stage 39 embryos stained for XlHbox8 expression (anterior towards the left), (panels 4–6) Ptf1a/p48 expression, (panels 7–9) insulin expression, (panels 10–12) XPDIp expression, (panels 13–15) Xhex expression. Red arrowheads demarcate the position of the ventral pancreas, white arrowheads the position of the dorsal pancreas. The statistics obtained are indicated at the right bottom corner of each panel. |
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pdia2 (protein disulfide isomerase family A, member 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35 & 36, lateral view, anterior left, dorsal up. |
