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Figure 2. Insm1 is required for endocrine cell differentiation. A-D: Insulin expression at both early (NF35; 90%, n = 15) and late stages (NF40/42; 79%, n = 52) is almost completely abolished. E-H: glucagon expression. I-L: somatostatin expression. At early stages expression of both gcg and som is reduced in the stomach/duodenum of insm1 knockdown animals (100%; n = 33 and n = 12, respectively), but begins to reappear at later stages (100%; n = 8 for both). The initial expression of these markers in the pancreas is also reduced at later stages (100% for gcg and 80% for som). M,N: Expression of the exocrine pancreas marker XPDIp is normal in insm1 morpholino injected tadpoles (100%, n = 10). O: In vitro transcription and translation of insm1 either alone (insm1), in the presence of the 5-bp mismatch morpholino (insm1+mMO) or in the presence of the morpholino (insm1+MO). Pictures presented in control are of uninjected embryos, but in all cases mismatch morpholino embryos (n ge 8 for each marker) gave identical results as control, with normal expression of these markers. The pancreas is outlined. |
