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Figure 1. PAPC is expressed in cranial neural crest (CNC) cells. (a) In situ hybridization (ISH) for PAPC and PCNS in tailbud embryos (stage 23). PCNS is expressed in the pharyngeal arches. PAPC transcripts are detected in the otic vesicle, but not in neural crest cells. (b) Quantitative real-time PCR exhibited PAPC expression in CNC explants. Explants were dissected at the indicated stage. All values are normalized to ODC expression. The expression of the CNC-specific marker genes twist, snail, and slug are shown for comparison. The bars indicate average and standard deviation of at least three independent experiments. |