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Fig. 4. XHRT1 early expression in the developing glomus is affected by translational inhibition of xWT1. (A) Design of the xWT1 MOs that target both pseudoalleles. (Bottom) In vivo translation of 500 pg of xWT1-eGFP is specifically inhibited by 15 ng of xWT1 MOs. (B-I) Control and injected sides of xWT1-depleted embryos stained with the indicated probes. xWT1 MOs abolished the expression of nephrin but had no effect on XSMP-30, Pax8 and Evi1. (J,K) Transversal sections of Evi1- and Pax8-stained xWT1-depleted embryos. Expression of both markers is unaffected by xWT1 knockdown. (L-O) Control and injected sides of xWT1-depleted embryos stained with XHRT1. Note that xWT1 knockdown decreases early but not late XHRT1 pronephric expression. |
