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Figure 2: Different levels of bleaching can be used to visualize staining in the embryo. (A) This blue staining along the bottom of this embryo shows the expression of hemoglobin in the ventral blood islands at about stage 36. This is a region of the embryo that is only lightly pigmented and thus the embryo was not bleached for a long time as can be seen by the tan colored pigment in the eye and along the flank of the embryo. Being able to see the pigmentation allows for better visualization of the stage of the embryo. If the staining is in a region with greater natural pigmentation, such as the nervous system and the flank of the embryo, greater bleaching will help view the in situ as seen in B. (B) Here the expression of pax8 in the forming kidney, pronephric duct and hindbrain is best visualized after bleaching has removed almost all endogenous pigment. Please click here to view a larger version of this figure. |
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pax8 (paired box 8 ) gene expression in Xenopus laevis embryo, NF stage 33 & 34, as assayed via in situ hybridization, lateral view, anterior left, dorsal up. |
