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FIG. 5. XPax-2 may supersede XPax-8 function later in pronephric development. In situ hybridization of stage 36 embryos with antisense
mRNA probes for XPax-8 (A), XPax-2 (B), and Xlim-1 (C). Note the even levels of expression throughout the tubules for XPax-8 compared
to the restriction of high levels of expression to the dorsal tips of the tubules for XPax-2 and Xlim-1. Also note that XPax-2 and Xlim-1 are
expressed in the nephric duct while XPax-8 is not. Embryos coinjected with XPax-2 and Xlim-1 mRNA show similar phenotypes to those
of embryos injected with XPax-8 and Xlim-1. D shows the injected side of a XPax-2 plus Xlim-1 coinjected embryo stained with the 3G8 antibody. Note the enlarged pronephros (boxed) compared to the uninjected side shown in E, indicating that XPax-2 and XPax-8 are functionally equivalent in this assay. |
