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Supplementary Figure 2: Expression of pronephric markers in wt and ruby mutant embryos. Embryos from a heterozygous carrier cross were fixed at 36-37 stage and the expression of pronephric markers: pax8 (paired box 8), pax2 (paired box 2), atp1a1 (ATPase, Na+/K+ transporting, alpha 1 polypeptide) , slc7a8 (solute carrier family 7 (amino acid
transporter light chain, L system), member 8), slc5a9 (solute carrier family 5
(sodium/glucose cotransporter), member 9), smp-30 (senescence marker protein-30), hnf1-b
(HNF1 homeobox b) and cdh16 (cadherin 16), were studied by WMISH. Embryos were genotyped to confirm the mutation after imaging. Arrowheads indicate abnormal pronephros. |