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Fig S5. Dose-dependent reduction of the kidney field induced by lhx1 depletion. Embryos were injected (1xV2) with different doses of lhx1-AS at the 8-cell stage. (A, E, F, H, I, K, L) In situ hybridization of embryos at stage 20 for the early pronephric marker pax8. (D, G, J, M) Visualization of the injected side (asterisk) by the presence of fluorescein dextran. (A) Uninjected embryo. (B) Control and injected (100 pg) sides of the same embryo are shown. (E) Control and injected (200 pg) sides of the same embryo are shown. (H) Control and injected (300 pg) sides of the same embryo are shown. (I) Reduction of pax8 expression was observed in 47% of the embryos and absence in 53% of the embryos (n = 34). (K) Control and injected (400 pg) sides of the same embryo are shown. |
