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Fig S3. Dose-dependent expansion of the kidney field induced by LL-VP16. Embryos were injected (1xV2) with different doses of LL-VP16 mRNA at the 8-cell stage. (A, E, F, H, I, K, L) In situ hybridization of embryos at stage 20 for the early pronephric marker pax8. (D, G, J, M) Visualization of the injected side (asterisk) by the presence of fluorescein dextran. (A) Uninjected embryo. (B) Control and injected (50 pg) sides of the same embryo are shown. (E) Control and injected (100 pg) sides of the same embryo are shown. (H) Control and injected (200 pg) sides of the same embryo are shown. (K) Control and injected (300 pg) sides of the same embryo are shown. (L) Expansion of pax8 expression was observed in 93% of the embryos (n = 27). Arrow indicates the misshapen kidney field.
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