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Fig S2. Dose-dependent expansion of the kidney field induced by LL-CA. Embryos were injected (1xV2) with different doses of LL-CA mRNA at the 8-cell stage. (A, E, F, H, I, K, L) In situ hybridization of embryos at stage 20 for the early pronephric marker pax8. (D, G, J, M) Visualization of the injected side (asterisk) by the presence of fluorescein dextran. (A) Uninjected embryo. (B) Control and injected (200 pg) sides of the same embryo are shown. (D) Expansion of pax8 expression was observed in 39% of the embryos (n = 31). (E) Control and injected (400 pg) sides of the same embryo are shown. (F) Expansion of pax8 expression was observed in 42% of the embryos (n = 33). (H) Control and injected (800 pg) sides of the same embryo are shown. (I) Expansion of pax8 expression was observed in 62% of the embryos (n = 34). (K) Control and injected (1200 pg) sides of the same embryo are shown. (L) Expansion of pax8 expression was observed in 82% of the embryos (n = 33). (N) Bar graph with the percentage embryos injected with the different doses of LL-CA that showed expansion of pax8 expression. |
