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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
crx
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
anterior neural fold
|
tbxt
|
|
laevis
|
NF stage 14
|
mesoderm
,
posterior
|
sox2
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
pax2
|
|
laevis
|
NF stage 17
|
trigeminal placode
,
eye primordium
,
neural plate
,
anterior neural fold
,
Rohon-Beard neuron
,
[+]
|
snai2
|
|
laevis
|
NF stage 17
|
branchial crest
,
neural crest
|
nog
|
|
laevis
|
NF stage 14
|
axial mesoderm
,
neural groove
,
anterior neural fold
|
tubb2b
|
|
laevis
|
NF stage 14
|
chordal neural plate
,
lateral
,
Rohon-Beard neuron
,
neuron
|
myod1
|
|
laevis
|
NF stage 14
|
mesoderm
,
presomitic mesoderm
,
paraxial mesoderm
|
|
|
Figure 4. Rbmx is necessary for normal neural development independent of neural induction and mesoderm formation. Embryos are either uninjected controls, injected with Rbmx MO in one cell at the one-cell stage (B,D,F,H,J,L) or one side at the two-cell stage Injected (N,M,P; injected side to the right). Embryos were analyzed at stage 14 (A-L) or 17 (M-R). A,B: N-tubulin-positive primary neurons do not form in Rbmx MO-injected embryos. C,D: Sox2 expression shows normal morphology of the neural plate. E-H: Early mesoderm formation is normal as judged by MyoD (E,F) and Xbra (G,H) expression. I,J: Otx5 expression in the anterior neural plate is not excluded from the presumptive forebrain. K,L: Noggin expression is absent in the anterior neural ridge (red arrow) indicating perturbed anterior neural development. M,N: Pax2-expressing interneurons in the spinal neural plate fail to form on the injected side. O,P: Reduced Slug expression on the injected side indicate defect in neural crest formation. Q,R: Mediolateral patterning of the spinal neural plate proper is normal judged by Pax6 expression. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
lhx1
|
|
laevis
|
NF stage 28
|
hindbrain
,
spinal cord
,
neuroectoderm
,
mesoderm
,
pronephric mesenchyme
,
[+]
|
snai2
|
|
laevis
|
NF stage 35 and 36
|
neural crest
,
melanophore
,
neural tube
|
rax
|
|
laevis
|
NF stage 31
|
otic vesicle
,
retina
,
pineal gland
|
en1
|
|
laevis
|
NF stage 28
|
midbrain-hindbrain boundary
,
neuroectoderm
,
neural tube
|
lbx1
|
|
laevis
|
NF stage 28
|
hindbrain
,
spinal cord
,
mesoderm
,
myotome
,
neural tube
,
[+]
|
|
|
Figure 5. Rbmx is required for normal eye development and neuronal differentiation. A-H: Embryos injected unilaterally with Rbmx MO and cultured to stage 31 (A-D), stage 28 (E-F), or stage 35 (H). A,B: In situ hybridization for Xrx1. C,D: In situ hybridization for Pax6; red lines indicate Pax6 expression domain in normal forebrain (C), which is absent in the Rbmx MO injected side (D). E-H: Dorsal view of head and anterior trunk of Rbmx morphants. Injected side is to the right. E-H: In situ hybridization using antisense probe for En1 (E), Lbx1 (F), Lim1 (G), and Slug (H). Red arrowhead in F indicates Lbx1 expression in hypaxial muscle on the uninjected side. Yellow line in H indicates the midline and red arrow indicates melanocytes on the uninjected side. Notably Slug expression is absent in the neural tube and trunk on the injected side. |