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Figure 6. Eye-specific marker genes are unaffected upon loss of Sox4 or Sox11 function.A and B: Depletion of Sox4 function had no influence on marker gene expression at stage 13. Loss of Sox11 led to a slight decrease of pax6 expression. C and D: At stage 23, Sox4 depleted embryos revealed a reduced expression of rax, pax6, sox3 and en2. Emx1 and egr2 expression was not affected. In contrast, all marker genes were mildly affected upon Sox11 knock-down. B and D. Quantitative representations are given. n = number of independent experiments, N = number of injected embryos analyzed, n.s. = not significant, * P≤0.05,** P≤0.01. |
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Figure 4. Proneural genes are not affected upon loss of Sox4 or Sox11.At stage 32, expression of proneural genes was not changed after loss of Sox4 or Sox11. Scale bar indicates 100 µm. |
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Figure 3. Sox4 depletion interferes with retinal lamination.Unilateral injection of 40 ng Sox4 or 40 ng Sox11 MO had no effect on the primary formation of most retinal cell types as shown by the expression of specific marker genes in mild eye phenotypes at stage 41. Only ganglion cells disappeared upon depletion of Sox4 or Sox11 in the severe eye phenotype. Many retinal cells were displaced (red arrowheads). Especially photoreceptor cells are displaced into inner layers of the retina (red arrowheads). In addition, the RPE is affected (black arrowheads). The uninjected (uninj.) sides revealed normal retinal lamination. For each marker gene, several embryos of different independent experiments were analyzed and showed a similar phenotype. Scale bar indicates 100 µm. |
