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Fig. 9. Effect of LPP3 in Xenopus axial patterning. (A-C) Effect of
murine LPP3 mRNA injection in Xenopus embryo development.
Stage 36 larvae (A) uninjected, (B) injected dorsally or (C) ventrally
with 1 ng of mLPP3 mRNA. Inserts show translated LPP3 protein.
Note that only the larvae injected dorsally had abnormal anterior
development. (D-H) In situ hybridization of un-injected and dorsally
injected Xenopus embryos with markers for anterior development
(stage 22). (D,E) Xotx2 detection in uninjected (bottom) and dorsally
injected (top) embryos. Some injected embryos lacked (D) or had
reduced (E) Xotx2 expression. Reduced and fused eyes can be
observed in the injected embryo (E, arrow). (F) Xpax6 detection in
uninjected (bottom) and dorsally injected (top) embryos. Injected
embryos lacked distinguishable eye staining. (G) Embryos injected
ventrally with Xwnt3a show a duplicated axis. (H) Co-injection of
mLPP3 with Xwnt3a mRNA rescues secondary axis formation but
results in a weak dorsalizalized phenotype. (I) Axis duplication
induced by ventral injection of Xwnt8. (J) Co-injection of Xwnt8 with
LPP3 mRNA inhibited axis duplication, but the embryos still
retained a weak dorsalization phenotype. |
