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Fig. 4. Retinal hyperplasia and ectopic expression of Xpax6 and Xrx as a result of Six6 overexpression. Serial sections of tadpole embryos (stage 36–38) after whole-mount RNA in situ analysis are shown in a rostral to caudal direction within each row; the injected side is to the right. Note the strong reduction or absence of RPE on the injected side (A–C) in Six6 injected embryos (100 pg). Xpax2 expression in the optic stalk is normal in the injected side (A,B), but expression is absent at the presumptive midbrain/hindbrain boundary (C). Xpax6 expression is broader in the optic area and expands into the presumptive midbrain and rostral hindbrain areas (D–F). Xrx expression is normally restricted to the ciliary margin of the retina. At the injected side of the embryo, Xrx expression extends up to the presumptive rostral hindbrain region (G–I). fb, forebrain; mb, midbrain; m/h, midbrain/hindbrain boundary; os, optic stalk; rpe, retinal pigment epithelium; cm, ciliary margin. |
