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pax6xenopus   

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Experiment details for pax6

Local translation of extranuclear lamin B promotes axon maintenance.

Local translation of extranuclear lamin B promotes axon maintenance.

Gene Clone Species Stages Anatomy
pax6.L laevis NF stage 40 to NF stage 45 retina , ciliary marginal zone , eye , retinal neural layer , central retina , [+]

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  Figure 3. LB2 mRNA and Protein Are Expressed in RGC Axons and GCs(A) Axonal lb2 mRNA detected by RT-PCR.(B and C) Lb2 FISH in cultured retinal axons and its quantitation (mean ± SEM; n = no. of GC; ∗p < 0.05; Mann-Whitney).(D–G′) ISH of stage 40–45 embryo sections (RGC: retinal ganglion cell layer; ONH: optic nerve head; and IPL/OPL: inner/outer plexiform layer). The same sections were counterstained for neurofilament and DAPI.(H and I) LB2 immunostaining in cultured retinal axons and its reduction by LB2MO.(J and K′) Immunostaining in tissue sections. The boxed areas are shown in the lower panels.Scale bars, 5 μm in (B) and (H), 25 μm in (D)–(G), (J), and (K), and 10 μm in (J) and (K) lower panels. See also Figure S3.