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pax6xenopus   

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Experiment details for pax6

Sumoylation controls retinal progenitor proliferation by repressing cell cycle exit in Xenopus laevis.

Sumoylation controls retinal progenitor proliferation by repressing cell cycle exit in Xenopus laevis.

Gene Clone Species Stages Anatomy
pax6.L laevis NF stage 18 optic vesicle , neural fold

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  Fig. S6. The effect of ubc9 and hmgb3 overexpression on eye field genes. (A-I) Xenopus embryos injected with synthetic RNAs, as indicated above each panel, were subjected to in situ hybridization with antisense RNA probes indicated on the left side of each panel.

Gene Clone Species Stages Anatomy
pax6.L laevis NF stage 32 to NF stage 33 and 34 forebrain , diencephalon , optic vesicle

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  Fig. S7. An enlarged eye phenotype begins to appear after stage 28. (A-O) Xenopus embryos injected with synthetic RNAs as indicated on the left side (A, B, E, F, I, J and C, D, G, H, K, L) were subjected to in situ hybridization with antisense RNA probes including rax (A-D), pax6 (E-H) and six3 (I-L). Injected side (A, C, E, G, I and K). Uninjected side (B, D, F, H, J and L). (M-O) Percentages of embryos that an eye region of the injected side is enlarged compared to uninjected side. Eye regions are delineated by the expression of rax (M), pax6 (N) and six3 (O). (lacZ: stage 28, n = 17 (M), n = 18 (N), n = 14 (O); stage 32/33, n = 18 (M), n = 22 (N), n = 20 (O); stage 35/36, n = 16 (M), n = 17 (N), n = 18 (O). lacZ/ubc9/hmgb3: stage 28, n = 30 (M), n = 22 (N), n = 29 (O); stage 32/33, n = 28 (M), n = 31 (N), n = 38 (O); stage 35/36, n = 24 (M), n = 23 (N), n = 26 (O))