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Fig. 6. Increasing Rx1 levels at stage 12 represses stem cell, neural identity and differentiation marker genes. (A) pax6 expression domain in the optic vesicle is smaller (arrow), and repressed in β-Gal labeled cells (inset, arrowheads). (B) In a small percentage of stage 38 embryos, pax6 expression is repressed in β-Gal labeled cells (arrowheads). (C) six3 expression domain in the optic vesicle is smaller (arrow). (D) In most stage 38 embryos, six3 expression is normal in β-Gal labeled cells (arrowheads). (E) notch1 expression domain in the optic vesicle is smaller (arrow), and repressed in β-Gal labeled cells (inset, arrowheads). (F) By stage 38 there is no repression surrounding β-Gal-labeled nuclei (arrowheads). (G) sox2 expression domain in the optic vesicle is smaller (arrow). (H) By stage 38 there is no repression surrounding β-Gal-labeled nuclei (arrowheads). (I) ngn1 expression in the optic cup (arrow) is fainter (cf. Fig. 5L) and repressed in β-Gal labeled cells (inset, arrowheads). (J) At stage 38, ngn1 expression continues to be repressed surrounding β-Gal-labeled nuclei (arrowheads). (K) p27 expression in the optic cup (arrow) is fainter (cf. Fig. 5N). (L) At stage 38, p27 expression continues to be repressed surrounding β-Gal-labeled nuclei (arrowheads) in some embryos. (M) neuroD expression in the optic cup (arrow) is greatly reduced (cf. Fig. 5P). (N) At stage 38, neuroD continues to be repressed surrounding β-Gal labeled nuclei (arrowheads). (O) Xath5 expression in the optic cup (arrow) is greatly reduced (cf. Fig. 5R). (P) At stage 38, Xath5 expression continues to be repressed surrounding β-Gal-labeled nuclei (arrowheads) in some embryos. Data for stage 16 activation are similar. Dashed lines indicate the outline of the retinas in the sectioned material. For whole mounts, bars = 100 μm; for sections bars = 20 μm. |
