|
Gene |
Clone |
Species |
Stages |
Anatomy |
pax6.L
|
|
laevis
|
NF stage 32
|
spinal cord
,
lateral ganglionic eminence
,
pallium
,
zona limitans intrathalamica
,
lateral pallium
,
[+]
|
pax6.L
|
|
laevis
|
NF stage 35 and 36
|
lateral ganglionic eminence
,
pallium
,
pretectum
,
prethalamus
,
zona limitans intrathalamica
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
eomes
|
|
laevis
|
NF stage 32
|
lateral ganglionic eminence
,
pallium
,
dorsal pallium
,
lateral pallium
,
medial pallium
,
[+]
|
eomes
|
|
laevis
|
NF stage 35 and 36
|
lateral ganglionic eminence
,
pallium
,
dorsal pallium
,
lateral pallium
,
medial pallium
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 32
|
brain
,
forebrain
,
telencephalon
,
diencephalon
,
optic stalk
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 35 and 36
|
telencephalon
,
optic stalk
,
hypothalamus
,
anterior hypothalamus
,
posterior hypothalamus
,
[+]
|
emx1
|
|
laevis
|
NF stage 32
|
diencephalon
,
dorsal pallidum
,
lateral pallium
,
medial pallium
,
pallidum
,
[+]
|
emx1
|
|
laevis
|
NF stage 35 and 36
|
diencephalon
,
pallium
,
zona limitans intrathalamica
,
thalamus
,
dorsal pallium
,
[+]
|
dlc
|
|
laevis
|
NF stage 32
|
brain
,
forebrain
,
telencephalon
,
diencephalon
,
lateral ganglionic eminence
,
[+]
|
dlc
|
|
laevis
|
NF stage 35 and 36
|
brain
,
forebrain
,
telencephalon
,
diencephalon
,
optic stalk
,
[+]
|
|
|
Fig.1. In toto analysis of x-Dll3, x-Nkx2.1, x-Emx1, x-Pax6 and x-Eomes expression in Xenopus brain. Whole-mount dissected brains at stage 32 (left column, entire brain) and stage 35 (right column, focused on the forebrain) are photographed in lateral views. In all panels, anterior is left and dorsal is up. The arrowhead in (A,C,E,G,I) indicates the position of the psb between the pallium (pa) and the subpallium (spa). The asterisk in (B) points to the x-Dll3 negative area in the rostral forebrain, see text. The inset indicates the plan of sections for transverse (t) and horizontal (h) sections in the next figures, as deduced from the axis of the brain. This antero-posterior axis is indicated by black arrows. Therefore, in the telencephalon, a horizontal section is parallel to the axis and allows the visualisation of transverse boundaries and divisions. Abbreviations are in Table 1. Scale BAR=100 μm. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
eomes
|
|
laevis
|
NF stage 32
to
NF stage 35 and 36
|
forebrain
,
telencephalon
,
diencephalon
,
pallium
,
dorsal pallium
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 32
to
NF stage 35 and 36
|
brain
,
forebrain
,
diencephalon
,
ventricular zone
,
medial ganglionic eminence
,
[+]
|
dlx2
|
|
laevis
|
NF stage 32
to
NF stage 35 and 36
|
forebrain
,
telencephalon
,
diencephalon
,
ventricular zone
,
lateral ganglionic eminence
,
[+]
|
emx1
|
|
laevis
|
NF stage 32
to
NF stage 35 and 36
|
forebrain
,
telencephalon
,
diencephalon
,
lateral ganglionic eminence
,
pallium
,
[+]
|
|
|
Fig. 2. Analysis on sections of x-Dll3, x-Nkx2.1, x-Emx1, x-Pax6 and x-Eomes expression at stage 32 and 35. (A–C,E,G) Single labelling and (D,F,H) double-labelling for the indicated genes. The gene names are colour-coded to indicate the pink, orange or purple reaction associated to the probe. All panels show horizontal sections (presented from rostral to caudal) and/or transverse sections (presented from dorsal to ventral). Asterisk in (A) indicates the x-Dll3 negative area surrounded by the subpallium (same as Fig. 1, panel B), discussed in text as the rostral pallium. In all panels, arrowheads indicate the presence of pallial markers expressed in a subpallial (x-Dll3 expressing) location, discussed in text as being the striatum. In all panels, arrows indicate pallial markers expressed in the rostral pallium, discussed in text as being the olfactory bulb. Asterisk in (F) (transverse section) shows the absence of x-Pax6 expression in the MZ of a subregion of the pallium, discussed in text as the DP. The results are pooled from stage 32 (A,B,D,E,G and Hhorizontal) and 35 (C,F and Htransverse) embryos. Note that there is no strict correspondence between the antero-posterior/dorso-ventral levels shown in the different panels. See Table 1 for abbreviations. Scale BAR=100 μm. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
eomes
|
|
laevis
|
NF stage 40
|
forebrain
,
telencephalon
,
pallium
,
dorsal pallium
,
lateral pallium
,
[+]
|
emx1
|
|
laevis
|
NF stage 40
|
medial ganglionic eminence
,
pallium
,
dorsal pallium
,
ventral pallium
,
prosomere
|
dlc
|
|
laevis
|
NF stage 40
|
forebrain
,
telencephalon
,
lateral ganglionic eminence
,
subpallium
,
medial ganglionic eminence
,
[+]
|
|
|
Fig. 3. Relative expression of x-Dll3, x-Emx1, x-Pax6 and x-Eomes at stage 40. Expression of pallial markers (blue) relative to x-Dll3 (orange) on horizontal (A,B) and transverse (C) sections through the forebrain of stage 40 embryos. Horizontal sections and transverse sections are presented in a rostro-caudal and dorso-ventral order, respectively. Asterisk in (A) indicates the x-Dll3 negative area surrounded by the subpallium (same as Fig.1 and Fig. 2). In all panels, arrowheads indicate the presence of pallial markers expressed in a subpallial (x-Dll3 expressing) location. See Table 1 for abbreviations. Scale BAR=100 μm. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nkx2-1
|
|
laevis
|
NF stage 40
|
forebrain
,
telencephalon
,
hypothalamus
,
medial ganglionic eminence
|
lhx5
|
|
laevis
|
NF stage 40
|
forebrain
,
telencephalon
,
pallium
,
pretectum
,
anterior tectum
,
[+]
|
lhx8
|
|
laevis
|
NF stage 40
|
ventricular zone
,
branchial arch
,
hypothalamus
,
anterior hypothalamus
,
medial ganglionic eminence
|
lhx8
|
|
laevis
|
NF stage 40
|
retina
,
ventricular zone
,
branchial arch
,
eye
,
hypothalamus
,
[+]
|
|
|
Fig. 4. Co-expression of Xenopus regionalisation genes and LIM-hd genes in the same forebrain subdivisions. Horizontal sections through the telencephalon and the diencephalon of stage 32 embryos double labelled for x-Pax6 and x-Lhx5 (A) and x-Nkx2.1 and x-Lhx7 (B). For example, x-Nkx2.1 and x-Lhx7 are co-localised in the mge and hypothalamus but not in branchial arches (ba). tec, tectum. See Table 1 for abbreviations. Scale BAR=100 μm. |