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Figure 6. xYAP expands pax3-expressing neural crest progenitors.(A) The pax3-expressing neural crest progenitor field (NCP) is darker, longer, and/or wider (bracket) on the xyap-injected side. Dorsal view, stage 15. (B) xYAP MO-mediated knockdown (40 ng) eliminated pax3 expression in both neural crest progenitors and hatching gland (HG) precursors. Addition of exogenous xyap (YAP MO + xyap) rescued pax3 expression in neural crest progenitors (NCP), but not in hatching gland. Dorsal views, stage 17. (C) xyap mRNA injection into the precursor blastomere of the intermediate mesoderm does not cause pax3 expansion on the injected side (left panel, dorsal view). Right panel (posterior view, dorsal is down) shows part of the lineage labeled clone (red) denoting the injected side. (D) tead1 mRNA injection (100 pg) expands pax3-expressing neural crest progenitors (NCP) at a moderate frequency. xyap mRNA injection (100 pg) rarely expands this population. In combination (tead1/xyap, 100 pg each), this population is expanded in nearly every embryo. The repression of the pax3-expressing hatching gland progenitors (HG) also was greatest when TEAD/xYAP were co-expressed. Dorsal anterior views at stage 16. |