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Fig. 1. Modulation of neural crest markers by noncanonical Wnt signaling. (A-H)Albino Xenopus embryos were injected animally with 0.5-1
ng Wnt11 (A-E) or dnWnt5/11 (F-H) mRNA at the four-cell stage, cultured until stage 14/16 and the expression of FoxD3 (A,F), Snail2 (B), Sox8 (C), Sox2 (D,H), Pax3 (E) and MyoD (G) analyzed by in situ hybridization. b-galactosidase is a lineage tracer (light blue) marking the injected side. Arrows point to altered marker expression. Dorso-anterior view is shown. (I) RT-PCR analysis of gene expression in neuralized animal caps. Animal caps were isolated from embryos at stage 9, injected with RNA as indicated, and cultured until stage 16/20 for RT-PCR analysis. Snail2, Twist, FoxD3, Sox8 and Pax3 are induced by Wnt signaling in the absence of significant changes in the pan-neural marker Sox3. no Wnt or Frizzled stimulation; uni, uninjected animal caps; WE, whole embryos; T, no reverse transcriptase. |