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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
dbx1
|
|
laevis
|
NF stage 16
|
telencephalon
,
chordal neural plate
,
neural plate
,
ventral
|
irx3
|
|
laevis
|
NF stage 16
|
neuroectoderm
,
neural plate
,
neural fold
,
cardiac neural crest
,
anterior neural ridge
|
nrp1
|
|
laevis
|
NF stage 16
|
neural plate
|
pax6
|
|
laevis
|
NF stage 16
|
neural plate
,
neural tube
|
pax2
|
|
laevis
|
NF stage 16
|
chordal neural plate
,
neural plate
,
neural tube
,
presumptive midbrain-hindbrain boundary
|
msx1
|
|
laevis
|
NF stage 16
|
ectoderm
,
preplacodal ectoderm
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
|
tubb2b
|
|
laevis
|
NF stage 16
|
neuroectoderm
,
trigeminal placode
,
neural plate
,
Rohon-Beard neuron
,
neural tube
|
krt12.4
|
|
laevis
|
NF stage 16
|
ectoderm
|
|
|
Fig. 2. Effect of Nkx6 mRNA injection on neural development. Gene expression patterns analyzed by whole mount in situ hybridization. Top row: uninjected control embryos. Middle row: embryos injected with Nkx6.1 mRNA. Bottom row: embryos injected with Nkx6.2 mRNA. Injected side is to the right and red stain indicate beta-galactosidase lineage tracer. All embryos are analyzed at stage 16, except embryos stained for Pax3 (V–X), which were analyzed at stage 17. (A–C) Pan-neural marker Nrp1 is expanded in Nkx6 injected embryos. (D–F) Epidermal marker cytokeratin-81 (CK81) is reduced in Nkx6 injected embryos. (G–I) Primary neurogenesis is inhibited by Nkx6 mRNA injected as visualized by loss of N-tubulin (N-tub) staining. (J–L) Dbx1 expression is inhibited in the spinal neural plate when embryos are injected with Nkx6 mRNA. (M–O) Spinal neural plate expression of Pax2 is repressed by Nkx6 mRNA, whereas the MHB is intact. (P–R) Spinal, but not rhombomeric, expression of Irx3 is repressed by Nkx6 misexpression. (S–U) Pax6 expression in the neural plate is repressed by ectopic Nkx6 misexpression. (V–X) Pax3 expression and Msx1 (Y–æ) expression is unchanged in response to Nkx6 injection. |