| |
Figure 1. Inhibition of proximal tubule formation in the pronephros by the loss of Prdx1 and its rescue by Prdx1* RNA co-injection. (A) prdx1 MOs (40 ng) were injected into both blastomeres of two-cell stage embryos. Embryos at the stage 33 were used for whole-mount in situ hybridization and immunohistochemistry. Proximal tubules in the pronephros were visualized by whole-mount in situ hybridization using smp30, xPteg, and pax2 probes and by immunohistochemistry using a 3G8 antibody. Expression of the proximal tubule-specific markers smp30 (B), xPteg (C), and pax2 (D) in prdx1 MO-injected embryos compared with the controls. Co-injection with prdx1* RNA rescued the decreased expression. The immunohistochemistry for 3G8 patterns were similar with whole-mount in situ hybridization (E). prdx1 morphants exihibited inhibition of proximal tubule formation only while intermediate, distal and connecting tubules were not affected by loss of prdx1.
|
