Search Criteria |
Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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pax2 | xenopus |
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Experiment details for pax2
CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus.CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus.
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Fig. 3. CRISPR injected embryos phenocopy established mutant or MO knockdown phenotypes in the F0 generation. Left panel=WT embryo, Middle panel=MO injected embryo, Right panel=CRISPRp injected embryo. (a) Foxj1 CRISPRp – Immunohistochemistry of stage 28 embryos with acetylated tubulin to mark surface cilia. (b) Pax8 CRISPRp – in situ hybridization of stage 38 embryos with pax2 probe to stain kidney tubule. Black arrow points to pronephros. |