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Figure 2. Partial depletion of Dzip1 increased the expression of Hh target genes in embryos. (A) In situ hybridization showing that partial Dzip1 depletion (right) markedly increased the expression of ptc1 in the neural tube and somites at stage 35. (B) Summary of the ptc1 expression phenotypes in control and morpholino injected embryos. DMO MM: 5bp-mismatch control morpholino of DMO2. (C) In situ hybridization showing the expression of floor plate markers (foxa2 and nkx2.2) and markers for more dorsal neural tube cells (pax2 and pax6) in controls (left) and embryos injected with DMO (right). The white dotted lines outline the neural tube borders. (D) Immunofluorescence showing total muscle (12/101 reactivity; top panel) and slow muscle cells (BA-F8 reactivity; bottom panel) in controls (left column) and DMO injected embryos (right column). Arrowheads denote ectopic BA-F8 reactive slow muscle cells evident in DMO injected embryos. (E) In situ hybridization showing eye patterning in controls (left) and DMO injected (right) embryos. Markers for optic stalk (pax2, top panel), total retina (rx, middle panel) and dorsal retina (brn3d, bottom panel) were analyzed. Dotted lines outline the eye. |
