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Fig. 1. Otx2 and Gbx2 form a boundary within the PPR. (A) Expression of Eya2 marks the PPR (bracket). (B)–(D) Double ISH for Otx2 (red) and Gbx2 (blue) at HH7. Lines indicate the level of sections shown in (C) and (D). (E)–(J) Parasagittal sections of stage HH5 (E)–(G) and HH7 (H)–(J) chick embryos after Gbx2 ISH (black (E), (F), (H), (I)) and Otx2 immunostaining (green; (F), (G), (I), (J)); DAPI labels nuclei ((G), (J); magenta). Arrows in (H)–(J) indicate anterior limit of Gbx2. (K) and (L) Double ISH in Xenopus for Otx2 ((K), (L); turquoise) and Gbx2 ((L); blue) at stage 12, dorsal to right anterior to the top. Red brackets indicate overlapping expression. (M) Diagram summarising expression of Otx2 and Gbx2 at stage 12 in Xenopus; red bracket: PPR. (N) Eya1 at stage 13 labels the PPR (bracket). (O) and (P) Double ISH for Otx2 ((O), (P); turquoise) and Gbx2 ((P); blue) at stage 13, dorsal to the right. Black brackets indicate the PPR. (Q) Diagram summarising neural plate and PPR expression of Otx2 and Gbx2 at stage 13 in Xenopus |
