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Fig. 4. PP2A:B56ϵ is required for MHB and anterior hindbrain gene expression in stage 16 neurulae. (A) The expression of otx2, en2, wnt1, krox20 and hoxB9 at the mid-neurula stage (stage 16) in control embryos (upper panels) and embryos injected with 2.5 ng PP2A:B56ϵ morpholino (lower panels) into both dorsal animal blastomeres at the 8-cell stage. For otx2, en2 and wnt1: anterior view; krox20 and hoxB9: dorsal view, with anterior upper most). (B) The expression of en2 and krox20 in embryos injected unilaterally with PP2A:B56ϵ morpholino (middle column), or PP2A:B56ϵ morpholino together with PP2A:B56ϵ-c mRNA (right column). All embryos were co-injected with nuclear β-galactosidase mRNA as a lineage tracer. Control (left column) is n-β-gal alone. |
