|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
hoxa1
|
|
laevis
|
NF stage 11.5
to
NF stage 12.5
|
circumblastoporal collar
|
hoxa1
|
|
laevis
|
NF stage 14
|
ectoderm
,
epidermis
,
neuroectoderm
,
chordal neural plate
,
floor plate
,
[+]
|
hoxa1
|
|
laevis
|
NF stage 26
|
midbrain
,
midbrain-hindbrain boundary
,
somite
,
pharyngeal arch
,
pronephric mesenchyme
|
hoxa1
|
|
laevis
|
NF stage 29 and 30
|
midbrain
,
midbrain-hindbrain boundary
,
spinal cord
,
somite
,
pronephric duct
,
[+]
|
hoxa1
|
|
laevis
|
NF stage 18
|
ectoderm
,
epidermis
,
chordal neural plate
,
neural tube
|
hoxd1
|
|
laevis
|
NF stage 11.5
to
NF stage 12.5
|
circumblastoporal collar
,
non-involuting marginal zone
,
posterior
|
hoxd1
|
|
laevis
|
NF stage 26
to
NF stage 29 and 30
|
pharyngeal arch
,
tail bud
|
hoxd1
|
|
laevis
|
NF stage 18
|
chordal neural plate
,
neural tube
|
hoxd1
|
|
laevis
|
NF stage 14
|
ectoderm
,
epidermis
,
chordal neural plate
,
posterior
|
hoxb1
|
|
laevis
|
NF stage 11.5
to
NF stage 12.5
|
neuroectoderm
,
circumblastoporal collar
,
non-involuting marginal zone
|
hoxb1
|
|
laevis
|
NF stage 18
|
neuroectoderm
,
neural plate
,
neural tube
,
rhombomere R4
|
hoxb1
|
|
laevis
|
NF stage 26
to
NF stage 29 and 30
|
pharyngeal arch
,
branchial arch
,
rhombomere R4
|
hoxb1
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
intermediate mesoderm
,
neural plate
,
rhombomere R4
|
|
|
Fig. 1. Hox PG1 genes have overlapping expression domains during Xenopus laevis development. Hoxa1 (A-H), Hoxb1 (I-P) and Hoxd1 (Q-X) have overlapping expression patterns. Gastrula stage embryos (A,B,I,J,Q,R) are shown from the vegetal side, dorsal up. When compared with Otx-2 expression (* in A,I,Q), it can be seen that the Hox expression does not reach the presumptive midbrain region. Neurula embryos are shown from the dorsal side, anterior to the right (C,E,K,M,S,U) or from the anterior side, dorsal up (D,F,L,N,T,V). Later embryos are shown from the lateral side, anterior to the right (G,H,O,P,W,X). Note pharyngeal arch expression of all three PG1 genes (arrows in G,H,O,P,W,X). |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nrp1
|
|
laevis
|
NF stage 22
|
forebrain
,
telencephalon
,
diencephalon
,
midbrain
,
hindbrain
,
[+]
|
egr2
|
|
laevis
|
NF stage 24
|
hindbrain
,
cranial neural crest
,
rhombomere R3
,
rhombomere R5
,
anterior branchial crest
|
gbx2.1
|
|
laevis
|
NF stage 22
|
hindbrain
,
midbrain-hindbrain boundary
,
otic placode
,
cranial neural crest
,
rhombomere
|
myod1
|
|
laevis
|
NF stage 22
|
somite
,
muscle
|
lsamp
|
|
laevis
|
NF stage 46
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
neural tube
,
[+]
|
|
|
Fig. 5. Triple PG1 knockdown severely affects hindbrain patterning. Morpholinos for each of the Hox PG1 genes (A1/B1/D1MO) were co-injected into the lhs of the embryo (B,D,F,H,J). Non-injected controls are also shown (A,C,E,G,I). The hindbrain region is shown, with anterior to the top. Expression of the neural marker Nrp1 (A,B: 100%, n=11), the anterior marker Otx2 (C,D: 100%, n=10), Gbx2, which is expressed in r1 (E,F: 77%, n=13) and Krox20 (G,H: no stripes 41%; one faint stripe 35%, n=71), was analysed. Dotted line indicates the midline. In later embryos (st. 46), the neural antibody 2-G9 was used to show the morphology of the hindbrain [NIC, I; A1/B1/D1MO, injected on the left-hand side (lhs) *, J: 100%, n=5]. Rhombomere boundaries are marked. |