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Fig. 2. Endodermal patterning occurs normally in BrunoL1 knockdown tadpoles. (A) In vitro transcription translation reactions. Translation of BrunoL1 is blocked in the presence of the antisense morpholino, but not the mismatch morpholino. (B,C) Ptf1a expression in control and BrunoL1 depleted tadpole showing normal induction of dorsal and ventral pancreatic buds (n=10). (Arrows point to the dorsal and ventral pancreatic buds that express Ptf1a.) (D,E) Hnf6 expression is also normal in BrunoL1 knockdown tadpoles (n=10). (F,G) Insm1 expression in the dorsal pancreas at stage 32 is normal in brunol1 morphants (n=10). (H,I) Expression of the endocrine progenitor marker insm1 (n=17) is reduced in both the pancreas and gastrointestinal tract. (J–M) Both neuroD (n=23) and pax6 (n=23) are also reduced, but some expression can be detected in the pancreas, most likely marking those beta cells that are present. Whole guts are from stages 41/42. In all panels the pancreas is outlined. (N,O) Trace drawings of panels J and K to illustrate the different
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