| |
Figure 5. Initial specification of the anterior endoderm is normal. A,B: Expression of ptf1a in the developing dorsal and ventral pancreatic buds was normal in insm1 knockdown tadpoles at NF35 (100%, n = 10). C,D: Expression of pdx1 in the pancreas and duodenum was unaffected (95%, n = 20). E,F: Initial expression of hnf6 in the anterior endoderm was also unaffected by the knockdown on insm1 (100%, n = 8). Pictures presented in control column are of uninjected embryos, but in all cases mismatch morpholino embryos (n = 15 for each marker) gave identical results as control, with normal expression of these markers. |
