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Fig. 3. Developmental expression patterns of Noelins. (A) Noelin temporal expression patterns. Embryos were harvested at the stages indicated and examined for the presence of the specific Noelin isoforms by RT-PCR. EF1a is a loading control, -RT is a control for genomic DNA contamination. Primer pairs used hybridize to only one splice variant each, as indicated. Noelin expression is observed weakly from neural plate stages, with strong expression from late neurula stages. (B-F) Spatial expression pattern of Noelin-3/4 (Y exon in situ hybridization, purple staining). Anterior is to the left. (B) Side view of stage 27 embryo with staining in cranial ganglia (arrowhead) and spinal cord; (C) stage 29 embryo with staining in trigeminal ganglion (arrowhead) and spinal cord; (D) anterior view of stage 26 embryo highlighting olfactory placode expression (arrowhead); (E) dorsal view of stage 42 embryo with staining in cranial ganglia, eye, and neuromasts; (F) magnification of boxed region in panel D showing neuromast expression (arrowhead). |
