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Fig. 5. Expression of XlaeOBP in Xenopus laevis olfactory cavities of tadpole at different stages of development analysed by ISH. Orientation: A, anterior; P,
posterior; R, right; L, left. (A) Stage 55, no XlaeOBP staining. (B) Stage 57, first stained cells appear (arrow). (C) Stage 59, the number of stained cells increases
(arrow); the open arrow indicates the duct between the vomeronasal organ and the PC. (D) Stage 60. (E and F) Stage 65; note that Bowman’s glands present their
mature morphology (arrow). |
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obp (olfactory binding protein) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 60, in sections of the olfactory epithelium. |
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Fig. 6. Expression of XtroOBP in adult Xenopus tropicalis olfactory cavities analysed by ISH. Orientation: A, anterior; P, posterior; R, right; L, left. (A) ISH with
XtroOBP antisense riboprobe led to labelling of Bowman’s gland (dotted circle) in the PC only. Asteriks indicate the valve that separates aquatic and aerial cavities.
(B) High magnification of the region labelled with XtroOBP antisense riboprobe; solid arrow, Bowman’s gland. Naturally pigmented cells, which characterize wildtype
animals, indicated by open arrow, are not riboprobe-stained cells. (C and D) Control experiment, hybridization of neighbouring sections with (C) sense and
(D) antisense probes. No staining was found with the sense probe. The dark cells are pigmented cells. |
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Fig. 3. Expression of XlaeOBP in adult Xenopus laevis olfactory cavity. Orientation: A, anterior; P, posterior; R, right; L, left. (A) ISH of Xenopus laevis with
XlaeOBP antisense riboprobe led to a labelling of Bowman’s glands in the PC only (arrows); no hybridization signal was found in MC. Asterisks indicate the valves
that separate aquatic from aerial cavities. (B) Hybridization of Xenopus laevis olfactory cavity with sense riboprobe; no signal was found. (C) High magnification
of the region labelled with XlaeOBP antisense riboprobe. An arrow indicates a duct opening from Bowman’s glands onto the principal (aerial) cavity. (D) ISH with
XlaeOBP antisense riboprobe at the VNO level. No staining is observed in the VNO. |
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Fig. 4. Expression of XlaeOBP restricted to the principal olfactory cavity. (A) Staining is located at the level of the Bowman’s gland (open arrow). (B) SDSPAGE
analysis of olfactory mucus selectively sampled from PC and MC of X. laevis. In the left panel, proteins were detected by Serva Blue G250 staining.
Molecular weights (M) with values are indicated. In the right panel, XlaeOBP was visualized in the PC by immunoblotting using polyclonal anti-XlaeOBP antibody.
No XlaeOBP was detected in the MC. |