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Figure 3. Results of combined antisense MoXat1 and MoXat3 injections in Xenopus embryos. Reduction of Xotx2 (A–C or J–L, respectively for strong or slight reduction), nrp-1 (D–F, strong; M–O, slight) and Twist (G–H, strong; P–Q, slight) expression is observed on the injected side of embryos (inj), compared to uninjected side (un). Strong or weak reduction (I, R respectively) of pharyngeal skeleton is observed on the injected side of antisense MO treated swimming tadpoles compared to control side. Beta-gal red staining traces injected side of embryos. |
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Figure S2. Results of antisense morpholino MoXat1 (A-I) or MoXat3 (J-R) injections in Xenopus embryos. Injections of 4 ng of single MO does not produce reduction in the expression of Xotx2 (A-C, J-L), nrp-1 (D-F; M-O) or Twist (G-H, P-Q) on the injected (inj) side of treated embryos compared to control (un) side. No reduction of pharyngeal skeleton is observed on the injected side in swimming larvae (I, R) compared to uninjected side. β-gal red staining traces injected side of embryos. |
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Figure S3. Results of standard control MO injections in Xenopus embryos. Injections of 8 ng of MO does not produce reduction in the expression of Xotx2 (A-C), nrp-1 (D-F) or Twist (G-H) on the injected (inj) side of embryos compared to control (un) side. No reduction of pharyngeal skeleton is observed on the injected side in swimming larvae (I) compared to uninjected side. β-gal red staining traces injected side of embryos. |
