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Fig. 1. Neural and mesoderm induction by FGF. In situ hybridization analysis on early gastrula stage treated ectoderm cultured to the early
tailbud stage. Untreated ectoderm (B,E,H), FGF-treated ectoderm (C,F,I), and the control whole embryos (A,D,G) were examined for
expression of nrp-1 (purple) and muscle actin (M. actin, blue) (A-C); otx2 (purple) and En-2 (blue) (D-F); and Hox B9 (G-I). FGF induces both
muscle actin and nrp-1, however, expression of the neural marker is not contingent upon muscle expression. The posterior marker, Hox B9, but
not the anterior markers, otx2 or En-2, is induced by FGF. In A, the stong expression of muscle actin obscures the nrp-1 expression in the
spinal cord. |
