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Fig. 3. Repression of nrp-1 expression by ENG-POU-GR. (A and B)
Whole-mount in situ hybridization of stage 20-22 embryos depicting
nrp-1 expression following the injection of ENG-POU-GR (60-300 pg)
mRNA into a dorsal animal blastomere at 8-16 cell stage (see Section 4).
Dex-treated embryos display a loss of neural tissue (A; b-gal staining,
depicting the injected side is turquoise). Non-dex-treated embryos show
normal patterns of expression for nrp-1 (B). Black arrows indicate injected
side. The alkaline phosphatase reaction product is purple. |