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nrp1xenopus   

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Experiment details for nrp1

Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification.

Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification.

Gene Clone Species Stages Anatomy
nrp1.L laevis NF stage 19 neural plate , anterior neural fold , neural tube

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  Fig. 4. Neural expansion in Xiro3-injected embryos. The injected side of the embryos is indicated and can be visualized by nuclear LacZ staining. Anterior is toward the right. Embryos are viewed from the dorsal or dorso-lateral side in (A), (B), (C) and (F) and from the the ventral side in (G) and (H). (A) Stage 30 embryo with increased XSox3 expression in the expanded neural tube. (B) Stage 15 embryo showing ectopic XSox3 expression in the ectoderm. (C) Ectopic expression of nrp-1 in the lateral ectoderm. (D) A section through the hindbrain region of a stage 30 embryo labelled for XSox3 expression. (E) A section through the hindbrain region of a Hoechst-stained stage 30 embryo showing that the number of cells in the expanded neural tube is increased. (F) X-twist is expressed in cranial neural crest on the control side but is absent on the injected side of the embryos. (G) Epidermal keratin is expressed outside the neural plate and is blocked in the Xiro3-injected area. (H) Like epidermal keratin, GATA-2 is expressed in the non-neural ectoderm and is absent after Xiro3 injection. (I) A section through the hindbrain of a stage 30 embryo showing that the formation of the somites as revealed by cardiac actin expression is affected on the injected side (n, notochord; s, somite).