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Fig. 6.Depletion of slc7a5 showed decrease ofpax6andnrl(L-maf) in eye development.Eye structure was investigated in slc7a5-depleted embryoby whole-mountin situhybridization withpax6andnrl(L-maf) probes. (A–D) Control MO-injected embryo. (A,B)pax6expression in control MO-injected embryo. (A)Uninjected side of control MO-injected embryo. (B)Injected side of control MO-injected embryo. (C, D)nrl(L-maf) expression in control MO-injected embryo. (C)Uninjected side of control MO-injected embryo. (D)Injected side of control MO-injected embryo. (E–H)slc7a5 MO-injected embryo. (E, F)pax6expression inslc7a5 MO-injected embryo. (E) Uninjected side of slc7a5MO-injected embryo. (F) Injected side of slc7a5 MO-in-jected embryo.pax6expression was repressed. (G, H)nrl(L-maf) expression in slc7a5 MO-injected embryo. (G)Uninjected side of slc7a5 MO-injected embryo. (H)Injected side of slc7a5 MO-injected embryo.nrl(L-maf)expression was decreased. (I–L) Rescue effect in coinjec-tion ofslc7a5.SmRNA andslc7a5.LmRNA with slc7a5MO. (I, J)pax6expression in rescue constructs-injectedembryo. (I) Uninjected side of rescue constructs-injectedembryo. (J) Injected side of rescue constructs-injectedembryo.pax6expression was rescued. (K, L)nrl(L-maf)expression in rescue contents-injected embryo. (K)Uninjected side of rescue constructs-injected embryo. (L)Injected side of rescue constructs-injected embryo.nrl(L-maf) expression was rescued. Yellow brackets indicatethe size of the eye.β-GalactosidasemRNA (1 ng) was usedas a tracer (blue). Scale bars: 1 mm. (For interpretation ofthe references to color in thisfigure legend, the reader isreferred to the web version of this article.) |
