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Fig. 4. Whole-mount in situ hybridization showing the expression of XL-maf. (A–F) Lateral view of whole embryos. (A′–F′) High magnification of the eye showing XL-maf expression (A–F). (A,A′) At stage 24 XL-maf is initially expressed in the PLE (arrowhead). (B,B′) At stage 28 staining is detected in lens placode. (C,C′) Stage 31. (D,D′) Stage 32. Strong signal in developing lens placode is found. (E,E′) Stage 35/36. XL-maf is expressed in lens detached from ectoderm. (F,F′) At stage 37/38 signal also appears in retina (arrowhead). |