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Figure 3. A-L: Xenopus nephrin expression examined by whole-mount fluorescent in situ hybridization. Anterior is to the left in all samples. A, B, and C are stage 26; D, E, and F are stage 28; G, H, and I are stage 33; J, K, and L are stage 36 embryos. A, D, G, and J are lateral views; B, E, H, and K enlargements of the panels to the left; and C, F, I, and L are dorsal views. |
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Figure 2. Xenopus nephrin is expressed in the pronephric glomus. A: Expression of nephrin (red arrow) in a stage 26 Xenopus laevis embryo assayed by in situ hybridization. B: Enlargement of A. C: Transverse section through a late tail bud stage embryo that had been stained previously for nephrin expression by in situ hybridization. Nephrin expression is medial to the region that will form the pronephric tubules (eosin counterstain). D: Double-stained stage 31 embryo. Somites 1-6 are stained light blue (immunohistochemistry with antibody 12/101) and nephrin (in situ hybridization) is stained brown. Somites 3-5 are indicated with blue arrows, and nephrin expression with a red arrow. E: Double-stained stage 35 embryo, as in D. Two head somites have disintegrated (dashed arrows) and another (4) partially disintegrated. |
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Figure 5. Three-dimensional anatomy of the glomus. The Xenopus glomera were visualized by nephrin fluorescent in situ hybridization, sampled by confocal microscopy, processed in Amira to generate voltex images, then surfaced and reimaged in 3D Studio. Both voltex and surface images are shown from anterior (dorsal is up) and dorsal views (anterior is up). Stages are shown to the left, and image type and views are shown above. Scale bars, in microns, are shown in the dorsal voltex view panels. |
