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Supplementary Fig. S7.Scp2 Morphant Phenotype. (A) Quantification of the whole mount immunostainings with 4A6 shown in Fig. 3K N. The embryos were categorized in three groups representing increasing severity of the phenotype. The amount of MOs injected and the number of embryos analyzed is indicated. (B-E׳) Whole mount in situ hybridization of uninjected and scp2-MO1+2-injected embryos for Nphs1 (B,B׳), Sglt1k (C,C׳), Nkcc2 (D,D׳) and Ncc (E,E׳) at stage 39. (F–H׳) Uninjected controls and scp2-MO1+2-injected embryos were stained with DAB to visualize endogenous peroxidase activity at stage 40 (F,F׳) and by whole mount in situ hybridization using the peroxisomal marker genes, Catalase (G,G׳) and Pmp70 (H,H׳) at stage 39. (I,I׳) Basigin whole mount in situ hybridization of uninjected controls and scp2 morphants at stage 39. |
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Supplementary Fig. S10. Pronephric Patterning in Xenopus Embryos Treated with Mevinolin. Whole mount in situ hybridization of untreated controls and embryos treated with 125 M Mevinolin for Nphs1 (A,A׳), Sglt1k (B,B׳), Nkcc2 (C,C׳) and Ncc (D,D׳) at stage 39. |