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Fig. 3. Analysis of pdzrn3 depletion effects using Na+/K+ATPase and nephrin pronephric markers. Xenopus embryos were injected with Mo-pdzrn3 at 2-cell stage into the marginal zone of one blastomere or at 16-cell stage into V1.1 blastomere, as indicated at the top of the injected side panels. In situ hybridization with Na+/K+ATPase probe on embryos at st. 37-38 (A-H) reveals that impairment of pdzrn3 gene function does affect the formation of pronephric proximal tubules (pt) but not the formation of the duct (A,C,E,G), or the formation of the glomus, revealed by in situ hybridization with nephrin probe (I,J). Scale bars: A,J, 300 mm. |