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nphs1 (nephrin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior right, dorsal up. |
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Fig. 3. Loss of Tbx2 function expands the territory of the pronephric duct and glomus but not the tubule. (A-L) Xenopus embryos injected with Tbx2 MO (10 ng), Tbx2δC-GR (200 pg) or control MO (Co MO, 10 ng) were subject to in situ hybridization for the glomus-specific markers WT1 and Nephrin and the duct-specific marker Gremlin at stage 35 or the tubule-specific marker SMP30 at stage 31. To activate injected Tbx2δC-GR mRNA, embryos were treated with DEX from stage 22 to 35 (B,E) or to 31 (H). (A-I) Stained embryos are shown in the upper part of each panel and transverse sections at the levels indicated by the dashed lines are shown below. Left and right parts of each panel show the injected and uninjected control sides, respectively. cl, coelom; g, glomus; pd, pronephric duct; pt, pronephric tubule. Arrows in A,B indicate WT1 and Nephrin expression in the somatic layer of the intermediate mesoderm, respectively. Arrows and bracket in D,E indicate migrating Gremlin-expressing cells and the diameter of pronephric duct, respectively. (J-L) Control MO has no effect on the expression of Nephrin, Gremlin or SMP30. |
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Fig. S4. Ectopic expression of WT1 expands the glomus domain and inhibits proximal tubule formation. (A-C) Embryos injected with WT1 RNA (200 pg) were subject to in situ hybridization for Pax2, Nephrin and Tbx2 at stage 32. Note that WT1 specifically impairs Pax2 expression in the pronephric tubules (arrowhead), whereas it increases Nephrin expression. Tbx2 expression is still detectable around the expanded pronephric mesoderm in the WT1-injected side of the embryo. Control, uninjected side. |