Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
nphs1xenopus   

Too many results?Too few results?

Experiment details for nphs1

Buisson I et al. (2014) Assay

Pax8 and Pax2 are specifically required at different steps of Xenopus pronephros development.

Gene Clone Species Stages Anatomy
nphs1.S laevis NF stage 33 and 34 to NF stage 35 and 36 glomus , pronephric kidney , kidney

Display additional annotations [+]
  Fig. 2. Pax 8 and Pax2 loss of function differentially affects tubule but not glomus marker genes expression at late tailbud stage. (A, B) Whole mount in situ hybridization for tubule marker genes. Embryos were injected with MoPax8 (ad) or MoPax2 (em) at the 4-cell stage, in the equatorial region of both left blastomeres. They were cultured until the tailbud stage 3335, fixed and analyzed by in situ hybridization with specific probes for the indicated genes. For each embryo, injected (am) and uninjected (am) sides are shown. Pax8 depletion prevents expression of marker genes all along the entire tubule expression domain. In Pax2 morphants, gene expression in the proximal part of the tubule, including nephrostomes, is affected. Expression in the distal tubule is not modified by the MoPax2 except for the terminal differentiation marker clcnkb1 whose expression is completely inhibited. Insets show higher magnification of the anterior pronephric territory. (C) Whole mount in situ hybridization for the glomus marker genes wt1 and nephrin. Embryos were injected with a mixture of MoPax8 and MoPax2 in the equatorial region of both left blastomeres at the 4-cell stage and cultured until the tailbud stage 33. Injected sides are shown in (n, o), uninjected sides in (n, o). Insets show higher magnification of the anterior pronephric territory. (D) RT-qPCR analysis of wt1 and nephrin. Embryos were injected with the indicated Mo at the 4-cell stage in all four blastomeres. They were cultured until the late tailbud stage 33 and processed for RT-qPCR analysis. Average values from three independent experiments. Neither MoPax2 nor MoPax8 injected alone or together significantly affects wt1 and nephrin expression compared to the control (MoC).

Gene Clone Species Stages Anatomy
nphs1.S laevis NF stage 33 and 34 to NF stage 35 and 36 glomus

Display additional annotations [+]
  Supplementary Material. Fig. S2 Pronephric phenotype induced by injection of MoPax2.2. Whole mount in situ hybridization for tubule and glomus marker genes. Embryos were injected with MoPax2.2 at the 4-cell stage, in the equatorial region of both left blastomeres. They were cultured until the tailbud stages 3335 (A) or 25 (B) and analyzed by in situ hybridization with specific probes for the indicated genes. For each embryo, injected (aq) and uninjected (aq) sides are shown. Pax2 depletion does not lead to any change in the expression of pronephric marker genes at stage 25. At the late tailbud stage, gene expression in the proximal part of the tubule, including nephrostomes, is affected by MoPax2.2 while expression in the distal tubule is not modified except for the terminal differentiation marker clcnkb1 whose expression is completely inhibited. Expression of the glomus marker genes wt1 and nephrin is not significantly affected in MoPax2.2 injected embryos. Insets show higher magnification of the anterior pronephric territory. Numbers indicate how many embryos showed the phenotype among the total number of injected embryos.

Gene Clone Species Stages Anatomy
nphs1.S laevis NF stage 33 and 34 to NF stage 35 and 36 glomus , pronephric kidney

Display additional annotations [+]
  Supplementary Material. Fig. S3 Pronephric phenotype induced by co-injection of MoPax8 and MoPax8.B. Whole mount in situ hybridization for tubule and glomus marker genes. Embryos were injected with MoPax8.B alone or in co-injection with MoPax8 at the 4-cell stage, in the equatorial region of both left blastomeres. They were cultured until the tailbud stages 3335 (A), early tailbud stage 2325 (B) and analyzed by in situ hybridization with specific probes for the indicated genes. For each embryo, injected (av) and uninjected (av) sides are shown. A strong reduction of the tubule marker genes (pax2, odf3, smp30, lhx1, hnf1b, pax2, hrt1, and evi1) expression is observed in response to the injection while the glomus markers wt1 and nephrin are still expressed. Insets show higher magnification of the anterior pronephric territory.