|
|
|
nphs1 (NPHS1 nephrin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 37 & 38, lateral view, anterior right, dorsal up, trunk region only.
|
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
slc5a1.2
|
|
laevis
|
NF stage 37 and 38
|
late proximal tubule
,
early proximal tubule
,
pronephric kidney
,
pronephric tubule
|
slc4a4
|
|
laevis
|
NF stage 37 and 38
|
late proximal tubule
,
late distal tubule
,
pronephric tubule
|
slc12a1
|
|
laevis
|
NF stage 37 and 38
|
early distal tubule
,
pronephric kidney
,
pronephric tubule
|
atp1b1
|
|
laevis
|
NF stage 37 and 38
|
pronephric duct
,
late proximal tubule
,
early distal tubule
,
early proximal tubule
,
late distal tubule
,
[+]
|
clcnkb
|
|
laevis
|
NF stage 37 and 38
|
pronephric duct
,
early distal tubule
,
late distal tubule
|
|
|
Figure 6. Xenopus golph2 is required for pronephros development. A.
Schematic diagram of the pronephros with a summary of the pronephric marker genes that were used (lateral view, anterior towards the left). At stage 38, the pronephros is subdivided into distinct segments: the glomus, proximal tubule (PT1, PT2, PT3), intermediate tubule (IT1, IT2), distal tubule (DT1, DT2) and pronephric duct (PD). B Expression of the marker genes; the orientations of panels B to M show a lateral view, the anterior towards the right; the orientations of panels Bto Mshow a lateral view, the anterior towards the left. A total of 32 ng of Control or golph2 morpholino was injected into a single ventral blastomere of 4-cell-stage embryos from the lateral region; whole-mount in situ hybridization analysis was performed on stage 38. (B Nephrin; (a) transverse section of the embryos in panels Band C (D atp1b1; (F SGLT-1K; (H ClC-K; (J NKCC2; (L NBC1. |