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Fig. 4. wnt4 acts downstream of the Notch pathway in the pronephros. (A-D) X. laevis embryos were injected as previously described and left to develop to stage 32, when in situ hybridisation detecting expression of nephrin and slc5a2 was performed. (A) Knockdown of wnt4 translation using a MO-inhibited nephrin expression. (B) Co-injection of notch-ICD mRNA and the wnt4 MO also reduces nephrin expression. Co-injection of su(H)DBM mRNA with wnt4 mRNA, causes ectopic glomus formation (white arrow, C) and proximal tubulogenesis (white arrow, D). Asterisk denotes injected side. |
