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Fig. 2. wnt4 overexpression induces the same phenotypes as injection of notch-ICD mRNA. (A-E) X. laevis embryos were injected as previously described. wnt4 mRNA was co-injected with beta-galactosidase mRNA to act as a lineage tracer [blue (A); red (B-E) staining on the injected side]. At stage 41, 3G8 is ectopic (white arrows) and 4A6 is reduced on the injected side (A). At stage 36, Na+K+ATPase (B) and slc5a2 (C) expression is reduced upon wnt4 overexpression and at stage 32 odf3 (D) and nephrin (E) expression is ectopic (white arrows). Asterisk denotes injected side. |