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Fig. 1. Mis-activating early pronephric Notch signalling caused ectopic medial pronephrogenesis. (A-J) X. laevis embryos were injected at the eight-cell stage into a V2 blastomere to target tissues including the presumptive pronephric region. notch-ICD mRNA or deltaSTU mRNA was co-injected with beta-galactosidase mRNA to act as a lineage tracer [blue (A,B); red (C-J) staining on the injected side]. At stage 41, 3G8 is ectopic (white arrows) and 4A6 is reduced in embryos injected with notch-ICD mRNA (A). deltaSTU reduces 3G8 staining (white arrow) but has no effect on 4A6 staining (B). At stage 36, Na+K+ATPase expression is reduced after overexpression of notch-ICD (C), but only the proximal domain of Na+K+ATPase expression is reduced upon deltaSTU overexpression (D). slc5a2 expression is inhibited after overexpression of notch-ICD (E) and deltaSTU (F). At stage 32, odf3 (white arrows, G) and nephrin (black arrow, I) expression is ectopic upon notch-ICD mRNA injection. odf3 (white circle, H) and nephrin (J) are inhibited by deltaSTU. Asterisk denotes injected side. |
