| |
Fig. 3. XAWP1 is required for neural crest induction. (A-I)
One blastomere of a four-cell stage embryo was injected
in the dorso-animal region with Co MO (5 ng), AWP1 MO1
(5 ng for B,C,E,F; 2 ng for G,I), AWP1 MO2 (20 ng) and
hAWP1 (2.5 pg) as indicated and then subjected to in situ
hybridization against Slug or Sox10 at stage 16. The injected
side was traced by lacZ staining. All embryos are shown in
dorso-anterior view with posterior to the top. (J) Four-cell
stage embryos were injected in the animal pole region with
the indicated combination of noggin (100 pg), XWnt8-CSKA
(100 pg), Co MO (40 ng), AWP1 MO1 (20 ng) and AWP1
MO2 (40 ng), and the animal caps were dissected at stage
9 and cultured to stage 16 for RT-PCR analysis. (K-P) Two blastomeres of a four-cell stage embryo were injected
in the dorsal-marginal region with Co MO (10 ng) or AWP1 MO1 (10 ng) and subsequently subjected to in situ
hybridization against Xbra at stage 10.5 or MyoD or Xnot at stage 14. Embryos are shown in vegetal view with
dorsal to the top (K,L) or in dorsal view with anterior to the top (M-P). |
