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Experiment details for not

Peyrot SM et al. (2011) Assay

A revised model of Xenopus dorsal midline development: differential and separable requirements for Notch and Shh signaling.

Gene Clone Species Stages Anatomy
not.L laevis NF stage 10.5 to NF stage 11 dorso-lateral marginal zone , dorsal marginal zone , upper blastopore lip , presumptive axial mesoderm
not.L laevis NF stage 14 pre-chordal neural plate , notochord , circumblastoporal collar , axial mesoderm , neural plate , [+]

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  Fig. S6. Analysis of floor plate markers reveals two separate populations of medial floor plate. In situ hybridization for indicated markers was performed at stage 10.5–11 (A, C, E, G, I, K, M) and neurula stages (st.14—B, D, F, J, L, N, O; st.16—H, P). Prime panels (B', etc.) show transverse bisections of matching neurula stages (notochord outlined in red). Markers expressed in the organizer (marked by asterisk) at gastrula stages are expressed throughout the dorsal midline and in a narrow domain in the floor plate (A–N') at neurula stages. Netrin expression begins at early neurula stages (st.14, O) in a wider domain than early markers (O', P' compared to B'–N'). We have designated the narrow, early-forming FP the 1° MFP and the wider, later-forming FP the 2° FP.